This study is focused on development of functionalized inorganic-organic nanofibrousmaterial with antibacterial activity for wound dressing applications. The nanofibers combining poly(vinyl alcohol) and silica were produced by electrospinning from the sol and thermally stabilized. ThePVA/silica nanofibers surface was functionalized by silver and copper nanoparticles to ensure antibacterial activity. It was proven that quantity of adsorbed silver and copper nanoparticles depends on process time of adsorption. According to antibacterial tests results, this novel nanofibrousmaterial shows a big potential for wound dressing applications due to its significant antibacterial efficiency.