Polymer based nanofibers using ethylene-co-vinyl alcohol (EVOH) were fabricated by electrospinning technology. The nanofibers were studied for potential use as dressing materials for skinwounds treatment. Properties closely related to the clinical requirements for wound dressing were investigated, including the fluid uptake ability (FUA), the water vapour transmission rate (WVTR), the bacteria control ability of nanofibers encapsulated with different antibacterial drugs, and Ag of various concentrations. Nanofibre degradation under different environmental conditions was also studied for the prospect of long term usage. The finding confirms the potential of EVOH nanofibers for wound dressing application, including the superior performance compared to cotton gauze and the strong germ killing capacity when Ag particles are present in the nanofibers.