Biodegradable poly(lactide-co-glycolide) (PLGA) antimicrobial nanofibers containing silver nanoparticles were prepared by electrospinning technique. Characterization of the Ag/PLGA nanofiber mats were determined by using SEM, TEM, WAXD and UV-vis spectrum. The antibacterial efficacy of the composites mats made of silver nanoparticles and PLGA fibers was measured. The antimicroorganization efficacy of Ag nanoparticles along with the biodegradability of the PLGA fibers can be combined in practical medical applications.