A novel biosensor was prepared on the basis of laccase, Nafion and TiO2 loaded copper and carbon composite nanofibers (TiO2/CuCNFs) obtained through electrospinning, carbonization and solvothermal treatment. The introduction of TiO2 apparently improved the electrocatalysis of the biosensor for the detection of hydroquinone. In addition, cyclic voltammetry analysis of the biosensor showed a pair of well-defined redox peaks, and revealed that the electrochemical behavior of laccase was a surface-controlled process. Chronoamperometry technique was also employed to investigate the biosensor. The results displayed a linear range from 1 mu M to 89.8 mu M, detection limit of 3.65 mu M (S/N = 3), a sensitivity of 24.6 mu A/mM, a high selectivity, as well as good repeatability and stability. The biosensor showed great promise in hydroquinone monitoring. (C) 2016 Elsevier B.V. All rights reserved.