Cu-0 nanoparticles (NPs)-doped TiO2 nanofibers (NFs) were prepared as an effective photodegradation of three azo dyes as well as an antimicrobial agent under visible light. Fabrication of one dimensional zerovalent Cu nanoparticles-doped TiO2 nanofibers with high aspect ratio have been successfully synthesized by simple and low cost; electrospinning technique followed by hydrothermal process. The photocatalytic activity of introduced nanofibers was evaluated by performing of three azo dyes. Catalytic NFs show a superior photodegaradation activity under visible light as well as good antimicrobial activity. Moreover, photocatalyst nanofibers appeared good stability, which was used for three cycles without regeneration. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.