Electrospinning provides a simple and versatile method for fabricating nanofiber mats. Electrospun fiber mat of well aligned and order architectures was often required for many applications. In this communication, biaxial orientation mats were electrospun by a novel collector consisting of two rotating disks with conductive edge. In addition, an auxiliary electrode was induced to focus the electrostatic field and force the fibers to align regularly. The biaxial orientation structure was formed with the variation of rotation speed without revolving the fiber mat during the electrospinning process, and the dates presented in this paper demonstrated that the degree of biaxial orientation strongly depended on the rotation speed. This simple method has potential applications in textile and electronic areas. Copyright (C) 2009 John Wiley & Sons, Ltd.