Rana chensinensis skin collagen/poly(L-lactide) (PLLA) composite scaffolds were first fabricated by electrospinning. The nanofibrous matrices were evaluated for their potential mechanical property as skin substitutes. The morphological and mechanical properties of the electrospun nanofibers were evaluated. The electrospun collagen/PLLA composite nanofibers showed the cross section had different shapes to pure collagen fiber. The addition of PLLA to collagen increased the mechanical property of the nanofibers, which were higher than those of pure collagen film, It is also expected that both the hydrogen bonding interaction and the fiber morphologies may play a crucial role in the mechanical properties of nanofiber filaments. Therefore, this work provides a promising approach to fabricate scaffolds for skin wound dressing. (C) 2014 Published by Elsevier B.V.