Electrospun Highly Ordered Mesoporous Silica-Carbon Composite Nanofibers for Rapid Extraction and Prefractionation of Endogenous Peptides
2019/11/27 21:19:20
Zhu, G. T., X. Chen, X. M. He, H. Wang, Z. Zhang and Y. Q. Feng
A simple method was developed for the preparation of ordered mesoporous silica-carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion-exchange capacity. On the basis of the size-exclusion effect of the mesopores and mixed-mode hydrophobic/ion-exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid-phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three-step elution strategy, followed by LC-MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum.
- Journal: Chemistry-a European Journal
- Volume: 21
- Issue: 11
- Pages: 4450-4456
- ISSN: 0947-6539
- DOI: 10.1002/chem.201406237
- Year: 2015
- Number:
- Type:
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